Journal: Circulation Research

Article Title: Spontaneous Atherosclerosis in Aged Lipoprotein Lipase–Deficient Mice With Severe Hypertriglyceridemia on a Normal Chow Diet

doi: 10.1161/circresaha.107.156554

Figure Lengend Snippet: Figure 2. Immunocytochemical detection of macrophage and VCAM-1 staining in aortic sinuses (200). Representative photomicrographs from the aortic sinus of 20-month-old mice stained with oil red O (ORO), filipin, a monoclonal rat antibody against Mac-3, a polyclonal rabbit antibody against VCAM-1, or a polyclonal rabbit antibody against -actin. Positive staining for Ch by filipin was bright against dark background in the fluoromicroscope, and those for Mac-3, VCAM-1, and -actin appeared in brown. These stainings identified foam cells of macrophage origin filled with Ch as the major cell type present in the aor- tic root lesions and increased expression of VCAM-1 on the endothelium.

Article Snippet: The sections were 1 Online Supplement X Zhang et al., Spontaneous atherosclerosis in aged LPL deficient mice... 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 immersed in 0.01M PBS (PH7.4) and incubated overnight at 4°C with either a monoclonal rat anti-Mac-3 antibody (Pharmingen), a polyclonal rabbit anti-VCAM-1 antibody (Santa Cruz) or a polyclonal rabbit anti-α-actin antibody (Zhongshan Bio Co, China).

Techniques: Staining, Expressing

Journal: Frontiers in Oncology

Article Title: Intrinsic Differences in Spatiotemporal Organization and Stromal Cell Interactions Between Isogenic Lung Cancer Cells of Epithelial and Mesenchymal Phenotypes Revealed by High-Dimensional Single-Cell Analysis of Heterotypic 3D Spheroid Models

doi: 10.3389/fonc.2022.818437

Figure Lengend Snippet: List of IMC antibodies.

Article Snippet: 175 Lu , pan-actin , D18C11 , 3175032D , Fluidigm , 1:50.

Techniques: